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US 2002/0165380 A1Published application
DNA encoding a mammalian receptor (fb41a) and uses thereof
OwnerJonathan A. Bardinventor
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OwnerJonathan A. Bardinventor
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This invention provides an isolated nucleic acid encoding a mammalian fb41a receptor, a purified mammalian fb41a receptor, vectors comprising isolated nucleic acid encoding a mammalian fb41a receptor, cells comprising such vectors, antibodies directed to a mammalian fb41a receptor, nucleic acid probes useful for detecting nucleic acid encoding a mammalian fb41a receptor, antisense oligonucleotides complementary to unique sequences of nucleic acid encoding a mammalian fb41a receptor, transgenic, nonhuman animals which express DNA encoding a normal or mutant mammalian fb41a receptor, methods of isolating a mammalian fb41a receptor, methods of treating an abnormality that is linked to the activity of the mammalian fb41a receptor, as well as methods of determining binding of compounds to mammalian fb41a receptors.
An isolated nucleic acid encoding a mammalian fb41a receptor.
An isolated nucleic acid encoding a human fb41a receptor analog.
A purified mammalian fb41a receptor protein.
A nucleic acid probe comprising at least 15 nucleotides, which probe specifically hybridizes with a nucleic acid encoding a mammalian fb41a receptor, wherein the probe has a unique sequence corresponding to a sequence present within one of the two strands of the nucleic acid encoding the mammalian fb41a receptor and are contained in plasmid FB41a (ATCC Accession No. 209449).
A nucleic acid probe comprising at least 15 nucleotides, which probe specifically hybridizes with a nucleic acid encoding a mammalian fb41a receptor, wherein the probe has a unique sequence corresponding to a sequence present within (a) the nucleic acid sequence shown in FIG. 1A-lB (Seq. I.D. No. 1) or (b) the reverse complement thereto.
A nucleic acid probe comprising a nucleic acid molecule of at least 15 nucleotides which is complementary to a unique fragment of the sequence of a nucleic acid molecule encoding a mammalian fb41a receptor.
A nucleic acid probe comprising a nucleic acid molecule of at least 15 nucleotides which is complementary to the antisense sequence of a unique fragment of the sequence of a nucleic acid molecule encoding a mammalian fb41a receptor.
A transgenic, nonhuman mammal comprising a homologous recombination knockout of the native mammalian fb41a receptor.
A process for identifying a chemical compound which specifically binds to a mammalian fb41a receptor which comprises contacting cells containing DNA encoding and expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with the compound under conditions suitable for binding, and detecting specific binding of the chemical compound to the mammalian fb41a receptor.
A process for identifying a chemical compound which specifically binds to a mammalian fb41a receptor which comprises contacting a membrane fragment from a cell extract of cells containing DNA encoding and expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with the compound under conditions suitable for binding, and detecting specific binding of the chemical compound to the mammalian fb41a receptor.
A process involving competitive binding for identifying a chemical compound which specifically binds to a mammalian fb41a receptor which comprises separately contacting cells expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with both the chemical compound and a second chemical compound known to bind to the receptor, and with only the second chemical compound, under conditions suitable for binding of both compounds, and detecting specific binding of the chemical compound to the mammalian fb41a receptor, a decrease in the binding of the second chemical compound to the mammalian fb41a receptor in the presence of the chemical compound indicating that the chemical compound binds to the mammalian fb41a receptor.
A process involving competitive binding for identifying a chemical compound which specifically binds to a mammalian fb41a receptor which comprises separately contacting a membrane fraction from a cell extract of cells expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with both the chemical compound and a second chemical compound known to bind to the receptor, and with only the second chemical compound, under conditions suitable for binding of both compounds, and detecting specific binding of the chemical compound to the mammalian fb41a receptor, a decrease in the binding of the second chemical compound to the mammalian fb41a receptor in the presence of the chemical compound indicating that the chemical compound binds to the mammalian fb41a receptor.
A method of screening a plurality of chemical compounds not known to bind to a mammalian fb41a receptor to identify a compound which specifically binds to the mammalian fb41a receptor, which comprises
A method of screening a plurality of chemical compounds not known to bind to a mammalian fb41a receptor to identify a compound which specifically binds to the mammalian fb41a receptor, which comprises
A process for determining whether a chemical compound is a mammalian fb41a receptor agonist which comprises contacting cells transfected with and expressing DNA encoding the mammalian fb41a receptor with the compound under conditions permitting the activation of the mammalian fb41a receptor, and detecting an increase in mammalian fb41a receptor activity, so as to thereby determine whether the compound is a mammalian fb41a receptor agonist.
A process for determining whether a chemical compound is a mammalian fb41a receptor antagonist which comprises contacting cells transfected with and expressing DNA encoding the mammalian fb41a receptor with the compound in the presence of a known mammalian fb41a receptor agonist, under conditions permitting the activation of the mammalian fb41a receptor, and detecting a decrease in mammalian fb41a receptor activity, so as to thereby determine whether the compound is a mammalian fb41a receptor antagonist.
A process for determining whether a chemical compound specifically binds to and activates a mammalian fb41a receptor, which comprises contacting cells producing a second messenger response and expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with the chemical compound under conditions suitable for activation of the mammalian fb41a receptor, and measuring the second messenger response in the presence and in the absence of the chemical compound, a change in the second messenger response in the presence of the chemical compound indicating that the compound activates the mammalian fb41a receptor.
A process for determining whether a chemical compound specifically binds to and inhibits activation of a mammalian fb41a receptor, which comprises separately contacting cells producing a second messenger response and expressing on their cell surface the mammalian fb41a receptor, wherein such cells do not normally express the mammalian fb41a receptor, with both the chemical compound and a second chemical compound known to activate the mammalian fb41a receptor, and with only the second chemical compound, under conditions suitable for activation of the mammalian fb41a receptor, and measuring the second messenger response in the presence of only the second chemical compound and in the presence of both the second chemical compound and the chemical compound, a smaller change in the second messenger response in the presence of both the chemical compound and the second chemical compound than in the presence of only the second chemical compound indicating that the chemical compound inhibits activation of the mammalian fb41a receptor.
A method of screening a plurality of chemical compounds not known to activate a mammalian fb41a receptor to identify a compound which activates the mammalian fb41a receptor which comprises:
A method of screening a plurality of chemical compounds not known to inhibit the activation of a mammalian fb41a receptor to identify a compound which inhibits the activation of the mammalian fb41a receptor, which comprises:
A method of treating an abnormality in a subject wherein the abnormality is alleviated by increasing the activity of a mammalian fb41a receptor which comprises administering to the subject an amount of a compound which is a mammalian fb41a receptor agonist effective to treat the abnormality.
A method of treating an abnormality in a subject wherein the abnormality is alleviated by decreasing the activity of a mammalian fb41a receptor which comprises administering to the subject an amount of a compound which is a mammalian fb41a receptor antagonist effective to treat the abnormality.
Dependencies are inferred from the claim text.