Skin substitutes and uses thereof
AssigneeStratech Corporation
InventorsPaul Barth Conrad, Cathy Ann-Rasmussen Ivarie, Lynn Allen-Hoffmann
Life of this patent
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Abstract
The present invention relates to in vitro cultured skin substitutes, and in particular to improved methods for organotypic culture of skin substitutes. In some embodiments, the dermal equivalent of the skin substitute is lifted to air interface of the culture prior to seeding with keratinocytes. In other embodiments, increased concentrations of collagen are used to form the dermal equivalent. In still other embodiments, optimized media are utilized to maintain the skin equivalents.
Claims63
A process for preparing a seeded dermal equivalent comprising:
- a. providing a dermal equivalent, keratinocytes, and culture media having an air interface;
- b. culturing said dermal equivalent in said culture media;
- c. lifting said dermal equivalent to said air interface to provide a lifted dermal equivalent;
- and
- d. seeding said dermal equivalent with said keratinocytes to provide a seeded dermal equivalent.
A process for preparing a skin equivalent comprising:
- a. providing a dermal equivalent, keratinocytes, and culture media having an air interface;
- b. culturing said dermal equivalent in said culture media;
- c. lifting said dermal equivalent to said air interface to provide a lifted dermal equivalent;
- d. seeding said dermal equivalent with said keratinocytes to provide a seeded dermal equivalent;
- e. and culturing said seeded dermal equivalent under conditions such that a skin equivalent is formed.
A composition comprising a growth chamber having a porous bottom surface and a side-wall, said porous bottom having thereon a skin equivalent wherein said dermal equivalent is substantially adhered to said side-wall of said growth chamber.
A composition comprising a dermal equivalent, wherein said dermal equivalent contains about 0.2 mg collagen per square centimeter of said dermal equivalent to 2.0 mg collagen per square centimeter of said dermal equivalent.
A composition comprising BSA, isoproterenol, camitine, serine, oleic acid, linoleic acid, arachidonic acid, α-tocopherol, ascorbic acid, and EGF.
Dependencies are inferred from the claim text.