DNA for expression of alpha 1-antitrypsin in methylotrophic yeast
OwnerJohn Lezdey, K. Anne Kronis +1inventors
InventorsJohn Lezdey, K. Anne Kronis, Darren Lezdey
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Abstract
Alpha 1-antitrypsin is prepared by growing methylotropic yeast transformants containing in their genome at least one copy of DNA encoding alpha 1-antitrypsin, in operational linkage with DNA encoding a signal sequence, which is effective for directing secretion of proteins from the host cells, DNA constructs and recombinant yeast strains used for the expression and secretion of alpha 1-antritrypsin are also provided. The fermentation medium requires a pH of 6.5 to 7.5.
Claims3
A process for producing biologically active alpha 1-antitrypsin peptides comprising culturing the cells for Pichia pastoris strain selected from the group consisting of KM71 and cassette that contains a sequence of nucleotides encoding an alpha 1-antitrypsin peptide and a promoter region from Pichia pastoris AOX1 gene operably linked to the sequence of nucleotides that encodes glycosylated alpha1-antitrypsin, said construct further comprising the DNA sequence of a vector comprising plasmid pGAPz or pPICZ in a medium having a pH of about 6.8
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