Transgenic mice containing DEZ orphan receptor gene disruptions
AssigneeDeltagen, Inc.
InventorThomas J. Brennan
Life of this patent
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Abstract
The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a DEZ receptor gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.
Claims20
A targeting construct comprising:
- (a) a first polynucleotide sequence homologous to a DEZ receptor gene;
- (b) a second polynucleotide sequence homologous to the DEZ receptor gene;
- and
- (c) a selectable marker.
A method of producing a targeting construct, the method comprising:
- (a) providing a first polynucleotide sequence homologous to a DEZ receptor gene;
- (b) providing a second polynucleotide sequence homologous to the DEZ receptor gene;
- (c) providing a selectable marker;
- and
- (d) inserting the first sequence, second sequence, and selectable marker into a vector, to produce the targeting construct.
A method of producing a targeting construct, the method comprising:
- (a) providing a polynucleotide comprising a first sequence homologous to a first region of a DEZ receptor gene and a second sequence homologous to a second region of a DEZ receptor gene;
- and
- (b) inserting a positive selection marker between the first and second sequences to form the targeting construct.
A cell comprising a disruption in a DEZ receptor gene.
A non-human transgenic animal comprising a disruption in a DEZ receptor gene.
A method of identifying an agent that modulates the expression of a DEZ receptor, the method comprising:
- (a) providing a non-human transgenic animal comprising a disruption in a DEZ receptor gene;
- (b) administering an agent to the non-human transgenic animal;
- and
- (c) determining whether the expression of DEZ receptor in the non-human transgenic animal is modulated.
A method of identifying an agent that modulates the function of a DEZ receptor, the method comprising:
- (a) providing a non-human transgenic animal comprising a disruption in a DEZ receptor gene;
- (b) administering an agent to the non-human transgenic animal;
- and
- (c) determining whether the function of the disrupted DEZ receptor gene in the non-human transgenic animal is modulated.
A method of identifying an agent that modulates the expression of DEZ receptor, the method comprising:
- (a) providing a cell comprising a disruption in a DEZ receptor gene;
- (b) contacting the cell with an agent;
- and
- (c) determining whether expression of the DEZ receptor is modulated.
A method of identifying an agent that modulates the function of a DEZ receptor gene, the method comprising:
- (a) providing a cell comprising a disruption in a DEZ receptor gene;
- (b) contacting the cell with an agent;
- and
- (c) determining whether the function of the DEZ receptor gene is modulated.
A transgenic mouse comprising a homozygous disruption in a gene comprising SEQ ID NO:1, or a homolog thereof.
Dependencies are inferred from the claim text.