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AssigneeBristol-Myers Squibb Company
InventorsRoss Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
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The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-β-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
An isolated nucleic acid that encodes the glutaryl 7-ACA amidase having the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid of between 10 and 1722 nucleotides, which hybridizes under stringent conditions either to a DNA molecule consisting of SEQ ID NO:1 or to a DNA molecule consisting of the complement of SEQ ID NO:1.
A glutaryl 7-ACA amidase having the following characteristics:
A method for preparing a glutaryl 7-ACA amidase from Pseudomonas diminuta BS-203, said method comprising
Dependencies are inferred from the claim text.