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US 2002/0160496 A1Published application
Isolated GRP94 ligand binding domain polypeptide and nucleic acid encoding same, and screening methods employing same
AssigneeDuke University
InventorsDaniel T. Gewirth, Christopher V. Nicchitta
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Abstract
An isolated GRP94 ligand binding domain polypeptide, an isolated polynucleotide encoding the same, and methods of using the same to identify modulators of Hsp90 proteins.
Claims14
An isolated GRP94 ligand binding domain (LBD) polypeptide comprising:
- (a) a polypeptide encoded by the nucleotide sequence of any one of SEQ ID NOs:3 or 5;
- (b) a polypeptide encoded by a nucleic acid molecule that is substantially identical to any one of SEQ ID NOs:3 or 5;
- (c) a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:4 or 6;
- (d) a polypeptide that is a biological equivalent of the polypeptide of any one of SEQ ID NOs:4 or 6;
- or
- (e) a polypeptide which is immunologically cross-reactive with an antibody that shows specific binding with a polypeptide of any one of SEQ ID NOs:4 or 6.
3 more elements
An isolated nucleic acid molecule encoding a GRP94 ligand binding domain (LBD) polypeptide, comprising:
- (a) a nucleotide sequence of any one of SEQ ID NOs:3 or 5;
- (b) an isolated nucleic acid molecule substantially identical to any one of SEQ ID NOs:3 or 5.
- (c) an isolated nucleic acid molecule which hybridizes to a nucleic acid sequence as set forth in any one of SEQ ID NOs:3 or 5 under stringent wash conditions, and which encodes a GRP94 LBD polypeptide;
- or
- (d) an isolated nucleic acid molecule differing from the nucleic acid molecule of (a), (b) or (c) above in nucleotide sequence due to the degeneracy of the genetic code, and which encodes a GRP94 LBD polypeptide encoded by the nucleic acid molecule of (a), (b) or (c) above.
2 more elements
A method for identifying a substance that modulates GRP94 LBD function, the method comprising:
- (a) isolating a GRP94 LBD;
- (b) exposing the isolated GRP94 polypeptide to a plurality of substances;
- (c) assaying binding of a substance to the isolated GRP94 polypeptide;
- and
- (d) selecting a substance that demonstrates specific binding to the isolated GRP94 LBD polypeptide.
2 more elements
A method of screening a plurality of compounds for a modulator of a GRP94 ligand binding domain polypeptide, the method comprising:
- (a) providing a library of test samples;
- (b) contacting a GRP94 ligand binding domain polypeptide with each test sample;
- (c) detecting an interaction between a test sample and the GRP94 ligand binding domain polypeptide;
- (d) identifying a test sample that interacts with the GRP94 ligand binding domain polypeptide;
- and
- (e) isolating a test sample that interacts with the GRP94 ligand binding domain polypeptide, whereby a plurality of compounds is screened for a modulator of a GRP94 ligand binding domain polypeptide.
3 more elements
Dependencies are inferred from the claim text.