Methods and host cells for improved cell culture
AssigneeImmunex Corporation
InventorsArvia E. Morris, Pranhitha Reddy
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Abstract
The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as to improve production characteristics.
Claims44
An eukaryotic host cell genetically engineered to express a gene for a protein of interest and an IGF-1-signaling pathway gene.
A method of producing a protein of interest, the method comprising culturing an eukaryotic host cell genetically engineered to express a gene for a protein of interest and an IGF-1-signaling pathway gene under conditions such that the protein of interest is expressed.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell to express a gene that encodes a protein of interest, and to express an IGF-1-signaling pathway gene.
A method of producing a mammalian cell line capable of growth in serum-free medium, the method comprising exposing cells that have been genetically engineered to overexpress an IGF-1 signaling pathway gene to serum-free medium, and isolating a cell line that grows in serum-free medium.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell to express a protein of interest, wherein the eukaryotic cell has been genetically engineered to express an IGF-1 signaling pathway gene.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell to express an IGF-1 signaling pathway gene, wherein the eukaryotic cell expresses a protein of interest.
Dependencies are inferred from the claim text.