Process for scaled -up production of recombinant proteins using transgenic plant suspension cultures
AssigneeThe Regents of the University of California
InventorsKaren A. McDonald, Alan P. Jackman, Melody M. Trexler
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Abstract
The present invention relates to methods for semi-continuous culture of plant cells in a nutrient medium. The pH of the medium is monitored during the cell culture as a way of monitoring the expression of a product of interest by the cells.
Claims17
A method for semi-continuous culture of plant cells in a nutrient medium, the method comprising monitoring pH of the medium to monitor expression of an expression product made by the cells, wherein the expression product is encoded by a polynucleotide under the control of an inducible promoter.
A method for production of a recombinant expression product using semi-continuous culture of transgenic plant cells comprising a heterologous expression cassette comprising a polynucleotide encoding the expression product operably linked to an inducible promoter, the method comprising the step of exchanging an induction medium with a growth medium when the pH of the medium is above 6.5.
Dependencies are inferred from the claim text.