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AssigneeToyo Boseki Kabushiki Kaisha
InventorsTakahide Kishimoto, Atsushi Sogabe, Shizuo Hattori, Masanori Oka, Yoshihisa Kawamura
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The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, homocysteine and the like, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteines.
A method for determining formaldehyde, which comprises bringing glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30%, glutathione and an oxidized coenzyme into contact with a sample, and analyzing a compound resulting from the enzymatic reaction.
A method for determining formaldehyde, which comprises bringing glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30%, glutathione, one compound selected from the group consisting of thio-NADs and thio-NADPs, and one compound selected from the group consisting of reduced NADs and reduced NADPs into contact with a sample to allow cycling reaction and analyzing changes in the amount of a compound due to the reaction.
A method for determining formaldehyde, which comprises bringing glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30%, glutathione, one compound selected from the group consisting of reduced thio-NADs and reduced thio-NADPs, and one compound selected from the group consisting of NADs and NADPs into contact with a sample to allow cycling reaction and analyzing changes in the amount of a compound due to the reaction.
A method for determining homocysteine, which comprises bringing transferase utilizing homocysteine and other compound as a pair of substrates and said other compound into contact with a sample and measuring the resulting compound.
Glutathione-dependent formaldehyde dehydrogenase having the following physico-chemical properties:
Dimethylglycine oxidase having the following physico-chemical properties:
A reagent kit for formaldehyde determination, which comprises at least buffer, glutathione, glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30% and a reagent for analyzing a compound produced by the enzymatic reaction.
A reagent kit for formaldehyde determination, which comprises at least buffer, glutathione, glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30%, one compound selected from the group consisting of thio-NADs and thio-NADPs, and one compound selected from the group consisting of reduced NADs and reduced NADPs.
A reagent kit for formaldehyde determination, which comprises at least buffer, glutathione, glutathione-dependent formaldehyde dehydrogenase having a ratio of reactivity with thio-NAD to reactivity with NAD of not less than 30%, one compound selected from the group consisting of reduced thio-NADs and reduced thio-NADPs, and one compound selected from the group consisting of NADs and NADPs.
A reagent kit for homocysteine determination, which comprises at least buffer, transferase utilizing homocysteine and other compound as a pair of substrates, said other compound, and a reagent for analyzing a compound produced by the enzymatic reaction.
A reagent kit for homocysteine determination, which comprises buffer, betaine, betaine-homocysteine methyltransferase, dimethylglycine oxidase and, where necessary, sarcosine oxidase, and a reagent for measuring hydrogen peroxide produced by the enzymatic reactions.
A reagent kit for homocysteine determination, which comprises betaine, betaine-homocysteine methyltransferase, dimethylglycine oxidase and, where necessary, sarcosine oxidase, and a reagent for determination of formaldehyde produced by the enzymatic reactions.
Dependencies are inferred from the claim text.