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AssigneeNippon Zoki Pharmaceutical Co., Ltd.
InventorsYoichi Fujii, Kaori Otake
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A monoclonal antibody specific to Nap was prepared by using a crude membrane fraction from a cell line which has a high affinity to Nef as an antigen. A cDNA clone which encodes Nap was obtained by screening a cDNA library of said cell line utilizing the specific antibody. The nucleotide sequence of this cDNA was elucidated whereupon the full amino acid sequences of human Nap was identified. Since the anti-Nap monoclonal antibody inhibits the binding of Nef to Nap, it can be used as a new therapeutic agent for AIDS. In addition, it can be applied for the diagnosis of AIDS by clarifying the relation between the expression of Nap and the development of AIDS. Nap can be utilized for binding experiments to Nef. It is useful for the development of novel therapeutic agents for AIDS based upon a mechanism of action which inhibits the attachment of both factors and development of new inhibitors against the onset of AIDS by a mechanism of action different from that of reverse transcriptase inhibitors or protease inhibitors.
A protein having an amino acid sequence represented by SEQ ID NO:1 and functionally active homologues thereof.
A DNA molecule which encodes a protein having an amino acid sequence represented by SEQ ID NO:1 or which encodes a protein which is a functionally active homologue of the protein having an amino acid sequence represented by SEQ ID NO:1.
An anti-Nef-attachable protein monoclonal antibody which has a high affinity to Molt-4 clone no.8 cells (human CD4+ T cell line) and U937 cells (human macrophage cell line) and does not bind or attach to Raji cells (human B cell line), BT-2 cells (human gliocyte cell line) and Gin-1 cells (human fibroblast cell line).
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