Bifidobacteria and siderophores produced thereby and methods of use
AssigneeRegents of the University of Minnesota
InventorDaniel J. O'Sullivan
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Abstract
The present invention provides isolated Bifidobacterium strains that secrete a siderophore, and methods of using such strains. The present invention also provides a siderophore from bifidobacteria, and methods of obtaining and methods of using such siderophores.
Claims40
An isolated Bifidobacterium having the characteristics of strain RecB1.
An isolated Bifidobacterium having the characteristics of strain RecB4.
An isolated Bifidobacterium having the characteristics of strain J1.
An isolated Bifidobacterium having the characteristics of strain J2.
An isolated Bifidobacterium having the characteristics of strain J4.
An isolated Bifidobacterium having the characteristics of strain P1.
An isolated Bifidobacterium having the characteristics of strain 6A.
An isolated Bifidobacterium having the characteristics of strain 6A.
A method for inhibiting the replication of a microbe in the gastrointestinal tract of an animal, comprising administering to an animal a Bifidobacterium that secretes a siderophore, and measuring the presence of the microbe in the gastrointestinal tract, where a decrease in the presence of the microbe in the animal after administration of the Bifidobacterium indicates inhibition of the replication of the microbe.
A method for treating a lactase deficiency, comprising administering to an animal a Bifidobacterium that secretes a siderophore, and detecting the presence of unabsorbed lactose in the gastrointestinal tract, where a decrease in the presence of unabsorbed lactose after administration of the Bifidobacterium indicates treatment of the lactase deficiency.
A method for establishing a Bifidobacterium flora in the gastrointestinal tract of an animal comprising administering to an animal a Bifidobacterium that secretes a siderophore, and measuring the presence of the Bifidobacterium in the gastrointestinal tract of the animal after administration.
A method for preventing the replication of microbes in a food, the method comprising adding to the food a Bifidobacterium that secretes siderophore.
A method for decreasing the risk of colon cancer comprising administering to an animal a Bifidobacterium that secretes a siderophore, and detecting the presence of aberrant crypt foci in the colon of the animal, where a lower number of aberrant crypt foci relative to an animal not administered the Bifidobacterium indicates a decrease in the risk of colon cancer.
A composition for inhibiting the replication of a microbe in the gastrointestinal tract of an animal, the composition comprising a Bifidobacterium that secretes siderophore.
A method for obtaining a siderophore from a Bifidobacterium, the method comprising incubating a Bifidobacterium under iron limited conditions, and isolating the siderophore.
A method for preparing a siderophore, the method comprising incubating a Bifidobacterium under iron limited conditions, and sterilizing the culture.
A composition comprising a siderophore obtained from a Bifidobacterium, wherein the composition is sterile.
An isolated siderophore obtained from a Bifidobacterium.
An isolated siderophore that binds Fe2+, the siderophore obtained from a Bifidobacterium.
A method for decreasing the amount of free iron in a composition, the method comprising adding to a composition a siderophore obtained from a Bifidobacterium.
A method for decreasing the amount of free iron in a composition, the method comprising adding to a composition an isolated siderophore obtained from a Bifidobacterium.
A method for inhibiting the replication of a microbe in a composition, the method comprising adding to a composition a siderophore obtained from a Bifidobacterium.
A method for inhibiting the replication of a microbe in a composition, the method comprising adding to a composition an isolated siderophore obtained from a Bifidobacterium.
A method for altering the expression of a siderophore in a Bifidobacterium, the method comprising incubating under iron limited conditions a Bifidobacterium that does not secrete a siderophore, and selecting for a Bifidobacterium that replicates in the iron limited condition.
Dependencies are inferred from the claim text.