Epidermal specific regulatory sequence
OwnerMartin Yanofsky, Allen Sessions +1inventors
InventorsMartin Yanofsky, Allen Sessions, Detlef Weigel
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Abstract
A novel, epidermal-specific regulatory sequence is provided which has been isolated from the 5′ end of a plant ML1 gene. This tissue-specific regulatory sequence, operably associated with a nucleic acid sequence expressing a product of interest, initiates and regulates the transcription of the nucleic acid sequence in the L1 layer of a plant epidermis in meristems and young primordia.
Claims33
An isolated epidermal specific regulatory nucleic acid, wherein operably associating said regulatory nucleic acid with a heterologous nucleic acid results in epidermal specific expression of said heterologous nucleic acid.
A transgenic plant cell comprising an epidermal specific regulatory nucleic acid operably associated with a heterologous nucleic acid, wherein a plant derived from said plant cell specifically expresses said heterologous nucleic acid in the epidermis.
A transgenic plant comprising an epidermal specific regulatory nucleic acid operably associated with a heterologous nucleic acid, wherein said heterologous nucleic acid is specifically expressed in the epidermal layer of the plant.
An expression vector comprising an epidermal specific regulatory nucleic acid operably associated with a heterologous nucleic acid.
A method of specifically expressing a heterologous nucleic acid in the epidermis of a plant comprising:
- transforming a plant with a nucleic acid construct comprising an epidermal specific regulatory nucleic acid operably associated with said heterologous nucleic acid;
- and
- selecting for plants that exhibit epidermal specific expression of said heterologous nucleic acid.
Dependencies are inferred from the claim text.