Process for producing and multiplying lymphocytes
AssigneeWinfried Albert
InventorsHERBERT JUNGFER, HEINRICH BARCHET, WINFRIED ALBERT
Life of this patent
Estimating the term…
Abstract
A process is disclosed for growing and/or multiplying lymphocytes in a cell culture medium which contains a lymphocyte growth factor, as well as aurintricarboxylic acid, cyclosporin and/or ascomycin. This process is particularly suitable to produce tumoricide killer T-cells.
Claims6
Process for the production of lymphocytes, wherein lymphocytes are cultured, multiplied and isolated in a cell culture medium which contains a lymphocyte growth factor as well as additionally aurintricarboxylic acid or a substance which binds to a cyclophilin and inhibits calcineurin in this complex.
Process for the production of a mixture of T cells (CD2+, CD3+) and NK cells (CD2+, CD3−, CD16+), wherein mononuclear blood leucocytes are cultured in a culture medium which contains a T cell growth factor as well as additionally aurintricarboxylic acid and/or a substance that binds to cyclophilin and inhibits calcineurin in this complex and the said T cells and NK cells are isolated after a culture period of at least 10 days.
Process for the production of a mixture of T cells (CD2+, CD3+) and NK cells (CD2+, CD3−, CD16+), wherein mononuclear blood leucocytes are cultured in a culture medium which contains a T cell growth factor and an apoptosis antagonist and the said T cells and NK cells are isolated after a culture period of at least 10 days.
Dependencies are inferred from the claim text.