Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing

An extraction device for mating with a carrier comprising:

• a carrier-receiving portion at a first end;
• and
• a conduit interconnected to the carrier-receiving portion;
• the conduit extending between the carrier-receiving portion and a second end;
• wherein the carrier-receiving portion is adapted to receive a carrier having a transfer film such that a reservoir is formed and at least a portion of the transfer film is disposed within the reservoir.

An extraction device for mating with a carrier comprising:

• a carrier-receiving portion at a first end;
• and
• a conduit interconnected to the carrier-receiving portion;
• wherein the carrier-receiving portion is adapted to receive a carrier to form a reservoir and further adapted to selectively cover at least a portion of the carrier.

An extraction system comprising:

• a carrier having a transfer film;
• and
• an extraction device being removably coupled to the carrier;
• the extraction device comprising a carrier-receiving portion interconnected to at least one conduit;
• the carrier-receiving portion being adapted to receive the carrier such that a reservoir is formed.

An extraction device for mating with a carrier comprising:

• a carrier-receiving portion at a first end;
• the carrier-receiving portion including a shoulder and at least one flange;
• the at least one flange extending from the shoulder;
• the carrier-receiving portion further including a landing portion having a landing surface;
• the landing surface defining an inner opening;
• and
• a conduit interconnected to the carrier-receiving portion at the inner opening;
• the conduit extending between the carrier-receiving portion and a second end;
• wherein the carrier-receiving portion is adapted to receive a carrier and to form a reservoir by the carrier contacting the landing portion.

An extraction device for mating with a carrier comprising:

• a carrier-receiving portion at a first end;
• the carrier-receiving portion including an inner surface, a recess, and a landing portion;
• the recess being interconnected with the inner surface and the landing portion;
• the landing portion forming a landing rim that is raised from the recess;
• the landing rim defining an inner opening;
• and
• a conduit interconnected to the carrier-receiving portion at the inner opening;
• the conduit extending between the carrier-receiving portion and a second end;
• wherein the carrier-receiving portion is adapted to receive a carrier and to form a reservoir by the carrier contacting the landing rim.

An extraction device for mating with a carrier comprising:

• a carrier-receiving portion at a first end;
• the carrier-receiving portion including an inner surface, a landing portion, and a reservoir forming surface;
• the landing portion being interconnected with the inner surface and the reservoir-forming surface;
• the reservoir forming surface being encompassed by the landing portion;
• and
• at least one conduit interconnected to the carrier-receiving portion;
• the conduit extending between the reservoir-forming surface and a second end;
• wherein the carrier receiving portion is adapted to receive a carrier and to form a reservoir by the carrier contacting the landing portion.

An extraction device for mating with a carrier comprising:

• a first surface;
• a second surface;
• an outer surface;
• and
• an inner surface;
• the first and second surfaces being interconnected by the outer and inner surfaces;
• the inner surface defining a conduit extending between the first surface and the second surface;
• wherein a reservoir is formed by the carrier being joined at the first surface such that the reservoir is defined by the carrier and the conduit.

An extraction device delivery system comprising:

• a base;
• and
• a locator connected to the base;
• wherein the locator includes at least one aperture adapted to receive at least one extraction device.

A method for extracting matter on a carrier comprising the steps of:

• providing a carrier having a transfer film;
• transferring matter to the transfer film;
• providing an extraction device;
• mating the carrier to the extraction device;
• forming a reservoir with the transfer film;
• providing fluid into the reservoir to extract matter from the transfer film;
• and
• removing the fluid from the reservoir.

A method of delivering at least one extraction device comprising the steps of:

• providing an extraction device delivery system comprising a base;
• and
• a locator connected to the base;
• wherein the locator includes at least one aperture adapted to receive at least one extraction device;
• providing at least one extraction device;
• disposing the at least one extraction device within the at least one aperture;
• providing a carrier having a transfer film;
• passing the carrier through the locator such that the transfer film contacts the at least one extraction device;
• adhering the carrier to the extraction device to form a carrier-extraction device combination;
• and
• removing the carrier-extraction device combination.

A method for extraction comprising the steps of:

• transferring matter to a carrier by microcapture;
• providing an extraction device having a carrier-receiving portion and at least one conduit;
• inserting the carrier into the carrier-receiving portion;
• forming a reservoir comprising at least one surface of the extraction device and at least one surface of the carrier;
• the reservoir being interconnected to the at least one conduit;
• introducing fluid into said reservoir via the at least one conduit to extract matter on the transfer film;
• and
• removing said fluid.