Nonhuman helper-dependent virus vector

A basic vector, in particular for producing a viral vector for gene transfer, comprising

• (a) a packaging sequence of a bacteriophage,
• (b) viral sequences comprising two inverted terminal repeats (ITRs) and one or more packaging sequences of a nonhuman adenovirus, where the viral sequences are not able to bring about helper-independent viral replication and packaging in a permissive cell line,
• (c) a cloning site for insertion of heterologous DNA and, where appropriate, a reporter gene cassette, which are located inside the viral sequences (b),
• (d) at least two cleavage sites for a restriction endonuclease which flank both sides of the viral sequences (b),
• (e) a bacterial origin of replication and
• (f) a bacterial selection marker gene, where sequences (a), (e) and (f) are located outside the sequence region flanked by the cleavage sites (d).

A viral gene transfer vector comprising the coat of a nonhuman adenovirus and genetic material which is packaged therein and which comprises

• (a) viral sequences comprising two inverted terminal repeats (ITRs) and one or more packaging sequences of a nonhuman adenovirus,
• (b) one or more nucleic acid sequences which code for peptides or polypeptides which are heterologous in relation to the nonhuman adenovirus, in operative linkage to expression control sequences and
• (c) where appropriate a noncoding DNA for size adjustment,
• where the genetic material is not able to bring about helper-independent viral replication and packaging in a permissive cell line.