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US 2001/0055811 A1Published application
Ir maldi mass spectrometry of nucleic acids using liquid matrices
AssigneeFranz Hillenkamp
InventorFRANZ HILLENKAMP
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Abstract
Mass spectrometry of large nucleic acids by infrared Matrix-Assisted Laser Desorption/Ionization (MALDI) using a liquid matrix is reported.
Claims66
A process for performing matrix assisted laser desorption/ionization (MALDI) of a nucleic acid in preparation for analysis by mass spectrometry, comprising the steps of:
- (a) depositing a solution containing the nucleic acid and a liquid matrix on a substrate, thereby forming a homogeneous, thin layer of a nucleic acid/liquid matrix solution;
- and
- (b) illuminating the substrate with infrared radiation, so that the nucleic acid in the solution is desorbed and ionized.
A process for analyzing a nucleic acid by mass spectrometry, comprising the steps of:
- (a) depositing a solution containing the nucleic acid and a liquid matrix on a substrate, thereby forming a homogeneous, thin layer of a nucleic acid/liquid matrix solution;
- (b) illuminating the substrate of (a) with an infrared laser, so that the nucleic acid is desorbed and ionized;
- and
- (c) mass separating and detecting the ionized nucleic acid using an appropriate mass separation and analysis format.
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Dependencies are inferred from the claim text.