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OwnerF.C. Thomas Allnutt, Colleen Mary Toole +1inventors
InventorsF.C. Thomas Allnutt, Colleen Mary Toole, John Peter Morseman
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This invention is directed to the utilization of the developing methods for molecular manipulation of cyanobacteria and red algae (and potentially cryptomonad algae) to express of phycobiliproteins and phycobiliprotein linker fusion proteins and their utilization as phycobiliprotein, phycobilisome and subassembly based reagents. In particular, the present invention relates to a method for a specific binding assay to determine a target moiety which is a member of a specific binding pair, and provides an improvement in the method comprising using a detectable label which is a fusion protein containing both a phycobiliprotein domain and another domain corresponding to a first member of a specific binding pair, where the fusion protein binds to a second member of the specific binding pair to provide a detectable labeled complex. The domain derived from the first member of the specific binding pair can be directly fused to the phycobiliprotein or phycobiliprotein linker domain or be separated by a spacer that allows correct folding of both domains.
In a method for a specific binding assay to determine a target moiety which is a member of a specific binding pair, the improvement comprising using a detectable label which is a fusion protein containing a phycobiliprotein domain and another domain corresponding to a first member of a specific binding pair, wherein the fusion protein binds to a second member of the specific binding pair to provide a detectable labeled complex.
In a method for biologic activity assays to determine a particular entity which induces a known biologic effect, the improvement comprising using a detectable label which is a fusion protein containing a phycobiliprotein domain and a second domain which undergoes the known biologic effect upon encountering the particular entity, wherein the known biologic effect induces a detectable change in the fusion protein.
A non-covalent complex comprising a first member of a specific binding pair non-covalently bound to a fusion protein containing a phycobiliprotein domain and a specific binding domain corresponding to a second member of the specific binding pair.
An isolated and purified fusion protein comprising a domain corresponding to a molecule of at least 2000 daltons molecular weight and a domain corresponding to a phycobiliprotein subunit.
A method of producing an isolated and purified fusion protein containing a phycobiliprotein domain and a non-phycobiliprotein domain, said method comprising
In a method for screening a combinatorial library to determine moieties which recognize a specific target molecule, the improvement comprising using a detectable label which is a fusion protein containing a phycobiliprotein domain and another domain corresponding to the one member of a random peptide library,
A method of producing an isolated and purified fusion protein containing a phycobiliprotein and phycobiliprotein linker domain and a nonphycobiliprotein molecule domain, said method comprising
An isolated and purified fusion protein comprising a domain corresponding to a nucleic acid binding domain and a domain corresponding to a phycobiliprotein and/or phycobiliprotein linker subunit.
Dependencies are inferred from the claim text.