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AssigneeThe Trustees of Columbia University in the City of New York
InventorJeannette Chloe Bulinski
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This invention provides a vector comprising an isolated nucleic acid which encodes a nucleic acid segment of interest linked to one or more nucleic acid segments encoding at least two Green Fluorescent Proteins. Provided is the method for detecting a nucleic acid segment of interest in a living cell which comprises: (a) transfecting the living cell with an isolated nucleic acid which encodes the nucleic acid segment of interest linked to at least two Green Fluorescent Proteins. Additional isolated nucleic acids different from the nucleic acid segment of interest may also be linked to at least two nucleic acid which encodes at least two molecules of Green Fluorescent Protein. (b) culturing the transfected cell in conditions permitting expression of Green Fluorescent Protein and the nucleic acid segment of interest; and (c) detecting the fluorescence of the Green Fluorescent Protein, thereby detecting a nucleic acid segment of interest in a cell.
An isolated nucleic acid comprising a nucleic acid segment of interest linked to one or more nucleic acid segments encoding at least two Green Fluorescent Proteins.
A protein conjugate containing at least two molecules of Green Fluorescent Protein and a protein of interest.
A method for detecting a nucleic acid segment of interest in a living cell which comprises:
A method for detecting a nucleic acid segment of interest in a living cell which comprises:
Dependencies are inferred from the claim text.