Carrier for immobilizing biologically active substance

A carrier for immobilizing a biologically active substance comprising a base material insoluble in solvents and a compound having a carbodiimide group bound to the surface of the base material via a covalent bond.

A method of producing a carrier for immobilizing a biologically active substance comprising a base material insoluble in solvents and having an isocyanate group on its surface,

• wherein said method comprises covalently binding a compound having (i) more than two isocyanate groups, (ii) more than one isocyanate groups and more than one functional groups other than an isocyanate group or (iii) more than one isocyanate groups and a halogen atom, to a functional group on the surface of the base material insoluble in solvents with leaving at least one free isocyanate group of said compound, said functional group is capable of covalently binding to an isocyanate group, a functional group other than an isocyanate group, or a halogen atom.

A method of producing a carrier for immobilizing a biologically active substance comprising a base material insoluble in solvents and a compound having a carbodiimide group bound onto the surface of the base material via a covalent bond,

• wherein said method comprises covalently binding a compound having (i) more than two carbodiimide groups or (ii) more than one carbodiimide groups and more than one functional groups other than a carbodiimide group, to a functional group on the surface of the base material insoluble in solvents with leaving at least one free carbodiimide group of said compound, said functional group is capable of covalently binding to a carbodiimide group or a functional group other than a carbodiimide group.

A method of immobilizing a biologically active substance, wherein said method comprises;

• contacting a biologically active substance that is reactive with an isocyanate group or a carbodiimide group with a carrier for immobilizing a biologically active substance comprising a base material insoluble in solvents and a compound having an isocyanate group or a carbodiimide group bound onto the surface of the base material via a covalent bond.

A method for assaying a biologically active substance, said method comprising;

• reacting a first substance which is biologically active and immobilized on a carrier with a second substance capable of specifically binding to the first substance,
• and detecting the second substance which is indirectly bound or not bound to the carrier via a bond between itself, to analyze the first substance or the second substance in a sample, wherein said carrier is the one for immobilizing a biologically active substance comprising a base material insoluble in solvents and a compound having an isocyanate group or a carbodiimide group bound onto the surface of the base material via a covalent bond, and said first substance is immobilized on the carrier via the isocyanate group or the carbodiimide group.