Serotype-specific probes for listeria monocytogenes
AssigneeUniversity of Hawaii
InventorsSOPHIA KATHARIOU, XIANG-HEI LEI
Life of this patent
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Abstract
Recombinant nucleic acids comprising region(s) of Listeria monocytogenes genome that are unique to an individual serotype and genomic cluster are provided. Also provided are oligonucleotide probes and primers derived from the recombinant nucleic acid sequences and methods for their use in the detection and identification serovar 4 and genomic cluster IIB strains.
Claims26
An isolated nucleic acid molecule comprising DNA having at least 80% sequence identity to (a) a DNA molecule having the sequence of SEQ ID NO:1, or (b) the complement of the DNA molecule of (a).
An isolated nucleic acid molecule that comprising DNA that hybridizes to (a) a DNA molecule having the sequence of SEQ ID NO:1, or (b) the complement of the DNA molecule of (a).
An isolated nucleic acid molecule that comprising (a) DNA encoding a polypeptide having the sequence of amino acid residues about 374 to about 2017 of (SEQ ID NO:1), or (b) the complement of the DNA of (a).
An isolated nucleic acid molecule that comprising (a) DNA encoding a polypeptide having the sequence of amino acid residues about 2031 to about 2975 of (SEQ ID NO:1), or (b) the complement of the DNA of (a).
An isolated nucleic acid molecule of at least about 10 nucleotides that hybridizes to (a) the nucleic acid sequence of SEQ ID NO:1, or (b) the complement of the nucleic acid sequence of (a).
An antibody which specifically binds to a polypeptide encoded by the nucleic acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.