Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
AssigneesBio Merieux, Affymetrix, Inc.
InventorsAli Laayoun, Duc Do, Charles G. Miyada
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Abstract
A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.
Claims29
A process for labeling with signal amplification a ribonucleic acid (RNA), comprising:
- fragmenting the RNA to form RNA fragments,
- fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of said RNA fragments, said terminal phosphate having been released during the fragmentation, and
- binding a plurality of labeling agents to said first ligand on each of a plurality of said fragments.
A labeled RNA fragment comprising at 3′ end a phosphate or a thiophosphate bearing a fluorescein bound to an anti-fluorescein antibody bearing at least one biotin, said antibody bound to a labeled streptavidin.
Dependencies are inferred from the claim text.