Methods and products for tumor immunotherapy

A method for in situ tumor vaccination of a subject, comprising:

• administering to a site of a tumor of a subject an effective amount for preventing tumor growth of a microparticle preparation containing IL-12, the microparticles of the microparticle preparation have an average particle size of between 10 nanometers and 10 microns.

A method for in situ tumor vaccination of a subject, comprising:

• administering to a site of a tumor of a subject an effective amount for preventing tumor growth of a microparticle preparation containing IL-12, the microparticle of the microparticle preparation having been prepared by phase inversion nanoencapsulation.

A method for in situ tumor vaccination of a subject, comprising:

• administering to a site of a tumor of a subject an effective amount for preventing tumor growth of a microparticle preparation containing IL-12, wherein the microparticle preparation is administered to the subject during or following a medical procedure to remove or kill the tumor cells.

A method for preventing tumor metastasis in a subject, comprising:

• administering to a site of a tumor of a subject in need thereof an effective amount for preventing tumor metastasis of a microparticle preparation containing IL-12.

A method for effecting tumor regression in a subject, comprising:

• administering to a site of a tumor of a subject in need thereof an effective amount for effecting tumor regression of a microparticle preparation containing IL-12.

A method for in situ tumor vaccination of a subject, comprising

• administering to a tumor of a subject a microparticle preparation containing an effective amount of IL-12 and a cytokine that augments antigen processing and presentation, wherein the effective amount of IL-12 and the cytokine that augments antigen processing and presentation results in a synergistic prevention of tumor cell growth.

A method for in situ tumor vaccination of a subject, comprising:

• administering to a site of a tumor of a subject an effective amount for preventing tumor growth of a microparticle preparation containing IL-12, wherein between about 0.1% and 20% of the IL-12 released from the microparticle preparation in vivo is bioactive.

A method for in situ tumor vaccination of a subject, comprising:

• administering to a site of a tumor of a subject an effective amount for preventing tumor growth of a microparticle preparation containing IL-12, wherein the microparticle preparation has an IL-12 release rate of between about 60 pg/μg of particle/day and 3400 pg/μg of particle/day.