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US 2001/0041173 A1Published application
Adenovirus-based methods, and cells, useful for the expression of nucleic acid sequences
OwnerFrank L. Graham, Martina Anton +1inventors
InventorsFRANK L. GRAHAM, MARTINA ANTON, MICHAEL A. RUDNICKI
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Abstract
This invention provides helper-dependent adenovirus cloning vectors and helper adenoviruses, and methods for making and using such preparations, wherein the helper adenoviruses contain recombinase target sites that are useful in reducing the level of contamination of helper virus in helper-dependent adenovirus vector preparations.
Claims4
A method for expressing nucleic acid sequences which comprises introducing into a host a helper-dependent adenovirus vector comprising:
- (i) a deletion of up to approximately 35,000 bp of the adenovirus genome but retaining sufficient left and right sequences including left and right ITRs and a packaging signal sufficient to support viral DNA replication and packaging;
- and
- (ii) a fragment or fragments of foreign DNA sequences of up to about 35,000 bp.
A cell expressing Cre recombinase and adenovirus E1.
A human cell expressing Cre recombinase.
Dependencies are inferred from the claim text.