Culture media for growing spirochetes
OwnerSteven E. Phillips, Hamid Moayad +1inventors
InventorsSteven E. Phillips, Hamid Moayad, Lida Mattman
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Abstract
Culture media for use in the growth of mutant bacteria, particularly for the growth of sub-types of bacteria, generally known as cell wall deficient organisms, especially spirochetes. A method for reliably and reproducibly culturing various forms of cell wall deficient organisms from the blood of ill patients, and particularly for reliably and reproducibly culturing spirochetes from the blood and other body fluids of patients with chronic Lyme Disease or with multiple sclerosis.
Claims30
An in vitro spirochete culture comprising:
- (a) living spirochetes;
- and
- (b) culture medium, wherein the culture medium comprises:
- (i) thioglycollate broth, wherein the concentration of thioglycollate is in the range of 0.05 weight % to 0.15 weight % of the culture medium;
- (ii) yeast extract, wherein the concentration of yeast extract is in the range of 0.5 weight % to 1.5 weight % of the culture medium;
- and
- (iii) sucrose, wherein the sucrose is present in the medium culture in an amount 5 weight % of the medium culture to 50 weight % of the culture medium
- wherein the culture medium has a pH in the range from 6.8-7.8.
A method for identifying the presence of a spirochete in a human, comprising:
- (1) obtaining a sample of blood or other body fluid from a human;
- (2) culturing the sample in a culture medium, wherein the culture medium comprises:
- (a) thioglycollate broth, wherein the concentration of thioglycollate is in the range of 0.05 weight % to 0.15 weight % of the culture medium;
- (b) yeast extract, wherein the concentration of yeast extract is in the range of 0.5 weight % to 1.5 weight % of the culture medium;
- and
- (c) sucrose, wherein the sucrose is present in the medium culture in an amount in the range of 5 weight % of the medium culture to 50 weight % of the culture medium;
- and
- (3) identifying the presence of the spirochete in the culture, wherein the identification of the spirochete in the culture identifies the presence of the spirochete in the human.
A method for identifying the presence of spirochetes in a mammal, comprising:
- (1) obtaining a sample of blood from a mammal in a container, such that the obtained blood sample contains ethylenediaminetetraacetate (EDTA), in a concentration sufficient to inactivate complement in the blood sample;
- (2) aging the blood sample, wherein the temperature is in the range of 0 degrees C to 41 degrees C, and
- (3) assessing the growth of spirochetes in the blood sample.
Dependencies are inferred from the claim text.