Genomic sequences for protein production and delivery
OwnerDouglas A. Treco, Michael W. Heartlein +1inventors
InventorsDouglas A. Treco, Michael W. Heartlein, Richard F. Selden
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Abstract
An isolated nucleic acid molecule that hybridizes under stringent conditions, or shares at least 80% sequence identity, with a defined genomic region upstream of the coding region of a FSHβ gene, and a DNA construct containing that nucleic acid molecule as a targeting sequence for homologous recombination.
Claims38
A DNA construct that alters expression of an endogenous FSHβ gene in a mammalian cell upon integration into the genome of the cell via homologous recombination, the construct comprising a targeting sequence containing at least 20 contiguous nucleotides from SEQ ID NO:4 or 5 and a transcriptional regulatory sequence.
An isolated nucleic acid comprising at least 20 contiguous nucleotides of SEQ ID NO:4 or its complement.
An isolated nucleic acid comprising at least 20 contiguous nucleotides of SEQ ID NO:5 or its complement.
An isolated DNA comprising a strand that comprises a nucleotide sequence that (i) is at least 100 nucleotides in length and (ii) hybridizes under highly stringent conditions with SEQ ID NO:4 or 5, or the complement of SEQ ID NO:4 or 5.
An isolated DNA comprising a strand that comprises a nucleotide sequence that (i) is at least 100 nucleotides in length and (ii) shares at least 80% sequence identity with a fragment of SEQ ID NO:4 or 5 having the same length as the nucleotide sequence.
Dependencies are inferred from the claim text.