Iron chelator delivery system
AssigneeGwathmey, Inc.
InventorJudith K. Gwathmey
Life of this patent
Estimating the term…
Abstract
An iron chelator delivery system is disclosed. The system comprises iron chelator and a lipid carrier, e.g., a liposome. Methods for preparing and administering the iron chelator delivery system are also provided.
Claims29
An iron chelator delivery system, comprising an iron chelator and a lipid carrier.
A method of preparing an iron chelator delivery system, comprising the steps of
- (a) combining a liposome with an iron chelator;
- and
- (b) extracting the iron chelator-encapsulated liposomes to form an iron chelator delivery system.
A method of preparing an iron chelator delivery system, comprising the steps of
- (a) dissolving phosphatidyl choline (PC) and cholesterol (Ch) in chloroform (CHCl3) to form an aqueous phase and an organic phase;
- (b) adding iron chelator to the aqueous phase;
- (c) vortexing the aqueous and organic phases;
- (d) evaporating the organic phase under a partial vacuum to form iron chelator-encapsulated liposomes;
- (e) extruding the liposomes through membrane filters;
- (f) removing the non-encapsulated iron chelator by centrifugation;
- and
- (g) extracting the iron chelator-encapsulated liposomes to form an iron chelator delivery system.
A method of preparing an iron chelator delivery system, comprising the steps of
- (a) drying a mixture of phosphatidyl choline (PC) and cholesterol (Ch) in CHCl3 and vacuum desiccated to form liposomes;
- (b) hydrating the liposomes by adding a solution of iron chelator;
- (c) vortexing the solution to form iron chelator-encapsulated liposomes;
- (d) extruding the liposomes through membrane filters
- (e) dialyzing the liposomes to purify the iron chelator-encapsulated liposomes thereby forming the iron chelator delivery system.
A method of treating iron-overload in a mammal in need of treatment, comprising administering to the mammal an iron chelator delivery system comprising an iron chelator and a lipid carrier so that treatment occurs.
A method of treating iron-overload in a mammal in need of treatment, comprising administering to the mammal an iron chelator delivery system comprising iron chelator and a liposome so that treatment occurs, wherein the concentration of the iron chelator is about 1 μM to about 100 mM, the size of the liposome is about 10 nM to about 10 microns, the liposome is dissolved in a pharmaceutically acceptable excipient prior to administration, and the liposome is administered for about 20 to about 30 minutes.
Dependencies are inferred from the claim text.