Hematopoietic cell culture nutrient supplement
AssigneeInvitrogen Corporation
InventorsJOHN P. DALEY, BARBARA M. DADEY, WILLIAM C. BIDDLE, MICHELLE G. WYSOCKI
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Abstract
The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and for differentiating hematopoietic cells.
Claims54
A serum-free, eukaryotic cell culture medium supplement comprising one or more ingredients selected from the group consisting of one or more antioxidants, one or more albumins or albumin substitutes, one or more lipid agents, one or more insulins or insulin substitutes, one or more transferrins or transferrin substitutes, one or more trace elements, and one or more glucocorticoids,
- wherein a basal cell culture medium supplemented with said supplement is capable of supporting the expansion of CD34+ hematopoietic cells.
A serum-free, eukaryotic cell culture medium supplement comprising one or more antioxidants, and one or more ingredients selected from the group consisting of one or more albumins or albumin substitutes, one or more lipid agents, one or more insulins or insulin substitutes, one or more transferrins or transferrin substitutes, one or more trace elements, and one or more glucocorticoids,
- wherein a basal cell culture medium supplemented with said supplement is capable of supporting the expansion of CD34+ hematopoietic cells.
A serum-free eukaryotic cell culture medium comprising one or more ingredients selected from the group consisting of one or more antioxidants, one or more albumins or albumin substitutes, one or more lipid agents, one or more insulins or insulin substitutes, one or more transferrins or transferrin substitutes, one or more trace elements, one or more glucocorticoids, one or more inorganic salts, one or more energy sources, one or more buffering agents, one or more pyruvate salts, one or more pH indicators, one or more amino acids, and one or more vitamins, wherein the medium is capable of supporting the expansion of CD34+ hematopoietic cells.
The serum-free, eukaryotic cell culture medium according to claim 41, wherein said medium is obtained by combining water and the ingredients of claim 46.
Dependencies are inferred from the claim text.