Nucleic acid assays employing universal arrays
OwnerAlex Chenchik, Grigoriy S. Tchaga +1inventors
InventorsAlex Chenchik, Grigoriy S. Tchaga, Peter N. Simonenko
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Abstract
Hybridization assays, as well as kits, primers and arrays for use in practicing the same, are provided. In the subject assays, a population of tagged target nucleic acids generated from a population of tagged gene specific primers is contacted with an array of tag complements under hybridization conditions and the presence of any resultant hybridized tag target nucleic acid-tag complement structures is detected. The subject arrays find use in a number of different applications, e.g. differential gene expression analysis.
Claims25
A hybridization assay comprising the steps of:
- (a) generating a population of tagged target nucleic acids from an initial sample of nucleic acids with a collection of a representative number of tagged gene specific primers;
- (b) contacting said population of tagged target nucleic acids with an array of tag complements immobilized on a solid support;
- and
- (c) detecting any resultant hybridization complexes on said array.
A kit for use in a hybridization assay, said kit comprising:
- (a) at least one of:
- (i) an array of distinct tag complements immobilized on the surface of a solid support;
- and
- (ii) a set of a representative number of distinct tagged gene specific primers;
- and
- (b) means for identifying the physical location on said array to which each distinct tagged gene specific primer hybridizes.
An array of distinct tag complements immobilized on a solid support, wherein said tag complements are members of a collection of tag-tag complement pairs in which the magnitude of any difference in hybridization efficiency between any two tag-tag complement pairs in said collection does not exceed about 10 fold.
A set of a representative number of distinct tagged gene specific primers comprising a tag domain and a primer domain, wherein said tag domains are members of a collection of tag-tag complement pairs in which the magnitude of any difference in hybridization efficiency between any two tag-tag complement pairs in said collection does not exceed about 10 fold.
Dependencies are inferred from the claim text.