Human dendriac and brainiac-3

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:

• (a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n1-294 of SEQ ID NO:2 and SEQ ID NO:10, where n1 is an integer in the range of −25 through 56;
• (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues −25-m1 of SEQ ID NO:2 and SEQ ID NO:
• 10, where m1 is an integer in the range of 292 through 294;
• (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n1-m1 of SEQ ID NO:2 and SEQ ID NO:10, where n and m are integers as defined respectively in (a) and (b) above;
• (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Dendriac amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203056 and 209627 wherein said portion excludes from 1 to about 81 amino acids from the amino terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203056 and 209627;
• (e) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Dendriac amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203056 and 209627 wherein said portion excludes from 1 to about 3 amino acids from the carboxy terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203056 and 209627;
• (f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Dendriac amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203056 and 209627 wherein said portion include a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above;
• (g) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n2-324 of SEQ ID NO:4 and SEQ ID NO:12, where n2 is an integer in the range of −28 through 80;
• (h) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues −28-m2 of SEQ ID NO:4 and SEQ ID NO:12, where m2 is an integer in the range of 316 through 324;
• (i) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n2-m2 of SEQ ID NO:4 and SEQ ID NO:
• 12, where n2 and m2 are integers as defined respectively in (a) through (d) above;
• a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Brainiac-3 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203451 and 209463 wherein said portion excludes from 1 to about 108 amino acids from the amino terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203451 and 209463;
• (k) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Brainiac-3 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203451 and 209463 wherein said portion excludes from 1 to about 8 amino acids from the carboxy terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203451 and 209463;
• and
• (l) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete Brainiac-3 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit Nos. 203451 and 209463 wherein said portion include a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above.

An isolated Dendriac and Brainiac-3 polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:

• (a) the amino acid sequence of the full-length Dendriac polypeptide having the complete amino acid sequence shown in SEQ ID NO:2 and SEQ ID NO:10 (i.e., positions −25 through 294 of SEQ ID NO:2 and SEQ ID NO:10);
• (b) the amino acid sequence of the full-length Dendriac polypeptide having the complete amino acid sequence shown in SEQ ID NO:2 and SEQ ID NO:10 excepting the N-terminal methionine (i.e., positions −24-294 of SEQ ID NO:2 and SEQ ID NO:10);
• (c) the amino acid sequence of the predicted mature Dendriac polypeptide having the amino acid sequence at positions 1-294 in SEQ ID NO:2 and SEQ ID NO:10;
• (d) the complete amino acid sequence encoded by the cDNA clone contained in the ATCC Deposit No. 203056 and in ATCC Deposit No. 209627;
• (e) the complete amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in the ATCC Deposit No. 203056 and in ATCC Deposit No. 209627;
• (f) the complete amino acid sequence of the predicted mature Dendriac polypeptide encoded by the cDNA clone contained in the ATCC Deposit No. 203056 and in ATCC Deposit No. 209627;
• (g) the amino acid sequence of the full-length Brainiac-3 polypeptide having the complete amino acid sequence shown in SEQ ID NO:4 and SEQ ID NO:12 (i.e., positions −28 through 324 of SEQ ID NO:4 and SEQ ID NO:12);
• (h) the amino acid sequence of the full-length Brainiac-3 polypeptide having the complete amino acid sequence shown in SEQ ID NO:4 and SEQ ID NO:12 excepting the N-terminal methionine (i.e., positions −27 through 324 of SEQ ID NO:4 and SEQ ID NO:12);
• (i) the amino acid sequence of the predicted mature Brainiac-3 polypeptide having the amino acid sequence at positions 1 through 324 in SEQ ID NO:4 and SEQ ID NO:12;
• (j) the complete amino acid sequence encoded by the cDNA clone contained in the ATCC Deposit No. 203451 and in ATCC Deposit No. 209463;
• (k) the complete amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in the ATCC Deposit No. 203451 and in ATCC Deposit No. 209463;
• and
• (l) the complete amino acid sequence of the predicted mature Brainiac-3 polypeptide encoded by the cDNA clone contained in the ATCC Deposit No. 203451 and in ATCC Deposit No. 209463.

An isolated polypeptide comprising an epitope-bearing portion of the Dendriac protein, wherein said portion is selected from the group consisting of:

• a polypeptide comprising amino acid residues from about Leu-30 to about His-38 of SEQ ID NO:2 and SEQ ID NO:10;
• a polypeptide comprising amino acid residues from about His-50 to about Ala-59 of SEQ ID NO:2 and SEQ ID NO:10;
• a polypeptide comprising amino acid residues from about Trp-64 to about Trp-70 of SEQ ID NO:2 and SEQ ID NO:10;
• a polypeptide comprising amino acid residues from about Met-208 to about Val-213 of SEQ ID NO:2 and SEQ ID NO:10;
• a polypeptide comprising amino acid residues from about Lys-224 to about Asp-230 of SEQ ID NO:2 and SEQ ID NO:
• 10;
• a polypeptide comprising amino acid residues from about Ile-246 to about Leu-252 of SEQ ID NO:2 and SEQ ID NO:10;
• and a polypeptide comprising amino acid residues from about Gly-273 to about Glu-278 of SEQ ID NO:2 and SEQ ID NO:10.

An isolated polypeptide comprising an epitope-bearing portion of the Brainiac-3 protein, wherein said portion is selected from the group consisting of:

• a polypeptide comprising amino acid residues from about Lys-l to about Ser-12 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Pro-16 to about Pro-25 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Leu-36 to about Arg-41 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Pro-58 to about Asp-64 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Ser-73 to about Ile-84 in SEQ ID NO:4 and SEQ ID NO:
• 12;
• a polypeptide comprising amino acid residues from about Thr-87 to about Pro-97 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Leu-161 to about Val-167 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Asp-179 to about Gln-185 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Leu-197 to about Lys-203 in SEQ ID NO:4 and SEQ ID NO:12;
• a polypeptide comprising amino acid residues from about Gly-275 to about Asp-284 in SEQ ID NO:4 and SEQ ID NO:12;
• and a polypeptide comprising amino acid residues from about Arg-296 to about Glu-301 in SEQ ID NO:4 and SEQ ID NO:12.

An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:

• (a) the nucleotide sequence of SEQ ID NO:6;
• (b) the nucleotide sequence of SEQ ID NO:7;
• (c) the nucleotide sequence of SEQ ID NO:8;
• (d) the nucleotide sequence of a portion of the sequence shown in FIGS. 2A and 2B (SEQ ID NO:3) wherein said portion comprises at least 50 contiguous nucleotides from nucleotide 150 to 650;
• (e) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), and (d) above.