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US 2001/0024795 A1Published application
Immunoassay technique using multispecific molecules
OwnerBan-an Khaw, Jagat Narulainventors
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OwnerBan-an Khaw, Jagat Narulainventors
Estimating the term…
The present invention relates to compositions and methods for detecting very low concentrations of a molecule in a mixture. The detection of the molecule comprises the steps of first contacting a sample with a multispecific molecule capable of binding at least two molecules including the molecule to be detected, wherein the molecule to be detected is bound by the multispecific molecule thereby forming a complex, and second contacting the complex with a second, different molecule which is linked via a polymer to multiple detection signaling molecules. The invention may also be practiced by administration of the multispecific molecule in vivo, to a host for the molecule to be detected, either with or without the bound polymer probe and thereafter, respectively, either detecting the signaling molecule on the probe, or administering the probe and allowing it to bind the multispecific molecule, followed by detection of the signaling molecule on the probe.
A method of detecting an antigen of interest in a sample comprising:
The method of claim 3, wherein said tumor antigen is associated with breast, prostate, brain, liver, kidney, colon, pancreatic, stomach, or lung cancer.
A method of imaging an antigen bearing structure in a patient, comprising:
A kit for detecting for detecting an antigen of interest in a sample, comprising:
A detectable complex comprising an antigen of interest, a multispecific molecule which is other than two monoclonal antibodies that are chemically cross linked, and a detection probe.
A detection probe comprising a polylysine backbone, about 18 or more horseradish peroxidase labels per mole of probe, and one or more binding moieties.
Dependencies are inferred from the claim text.