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US 2001/0024787 A1Published application
Compositions, kits, and methods for hydrodynamic stress-related regulation of gene expression
AssigneeThe Trustees of the University of Pennsylvania
InventorsPeter F. Davies, Denise C. Polacek
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Abstract
Compositions, kits, and, methods for identifying genes, expression of which is spatially regulated in response to phenomena related to cellular hydrodynamic stress are disclosed.
Claims26
A method of identifying a gene, expression of which is regulated by hydrodynamic stress, the method comprising
- a) subjecting a first vascular endothelial cell of a mammal to hydrodynamic stress;
- b) thereafter assessing the level of expression of the gene in the cell, and
- c) comparing the level of expression of the gene in the first cell with the level of expression of the same gene in a second vascular endothelial cell of the mammal, the second cell being subjected to different hydrodynamic stress, whereby a difference between the level of expression of the gene in the first cell and the level of expression of the gene in the second cell is an indication that the gene is regulated by hydrodynamic stress.
A method of identifying a nucleic acid comprising a hydrodynamic stress regulation (HSR) region, the method comprising
- a) subjecting a first vascular endothelial cell of a mammal to hydrodynamic stress;
- b) thereafter assessing the level of expression of the nucleic acid in the cell, and
- c) comparing the level of expression of the nucleic acid in the first cell with the level of expression of the same nucleic acid in a second vascular endothelial cell of the mammal, the second cell being subjected to different hydrodynamic stress, whereby a difference between the level of expression of the nucleic acid in the first cell and the level of expression of the nucleic acid in the second cell is an indication that the nucleic acid comprises a HSR region.
A method of identifying a nucleic acid comprising an HSR region, the method comprising
- a) comparing the sequence of a first nucleic acid with the sequence of a second nucleic acid comprising an HSR region, and
- b) identifying a region of the first nucleic acid which is homologous to the HSR region of the second nucleic acid, whereby a nucleic acid comprising a HSR region is identified.
A method of identifying a hydrodynamic stress responsive protein, the method comprising
- a) subjecting a first vascular endothelial cell of a mammal to hydrodynamic stress;
- b) thereafter assessing the level of expression of a nucleic acid in the first cell, and
- c) comparing the level of expression of the nucleic acid in the first cell with the level of expression of the same nucleic acid in a second vascular endothelial cell of the mammal, the second cell being subjected to different hydrodynamic stress, whereby a difference between the level of expression of the nucleic acid in the first cell and the level of expression of the nucleic acid in the second cell is an indication that the nucleic acid encodes a hydrodynamic stress responsive protein, and
- d) identifying a protein encoded by the nucleic acid, whereby a hydrodynamic stress responsive protein is identified.
1 more element
Dependencies are inferred from the claim text.