Nucleic acid labeling compounds
OwnerGlenn H. Mcgall, Anthony D. Baroneinventors
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Abstract
Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.
Claims60
A nucleic acid labeling compound of the following structure:
- 42
- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is amido alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
A nucleic acid labeling compound of the following structure:
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- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is amino alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
A nucleic acid labeling compound of the following structure:
- 44
- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is alkynyl alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
A nucleic acid labeling compound of the following structure:
- 45
- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is amino alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
A nucleic acid labeling compound of the following structure:
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- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is functionalized alkyl, alkenyl alkyl or alkynyl alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
A nucleic acid labeling compound of the following structure:
- 47
- wherein A is H or a functional group that permits the attachment of the nucleic acid labeling compound to a nucleic acid;
- X is O, S, NR1 or CHR2, wherein R1 and R2 are, independently, H, alkyl or aryl;
- Y is H, N3, F, OR1, SR1 or NHR1, wherein R1 is H, alkyl or aryl;
- Z is H, N3, F or OR1, wherein R1 is H, alkyl or aryl;
- L is functionalized alkyl, alkenyl alkyl or alkynyl alkyl;
- Q is a detectable moiety;
- and, M is a connecting group, wherein m is an integer ranging from 0 to about 3.
Dependencies are inferred from the claim text.