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AssigneeRosetta Inpharmatics LLC
InventorsStephen H. Friend, Roland Stoughton
Estimating the term…
The present invention provides methods for monitoring disease states in a subject, as well as methods for monitoring the levels of effect of therapies upon a subject having one or more disease states. The methods involve: (i) measuring abundances of cellular constituents in a cell from a subject so that a diagnostic profile is obtained, (ii) measuring abundances of cellular constituents in a cell of one or more analogous subjects so that perturbation response profiles are obtained which correlate to a particular disease or therapy, and (iii) determining the interpolated perturbation response profile or profiles which best fit the diagnostic profile according to some objective measure. In other aspects, the invention also provides a computer system capable of performing the methods of the invention, data bases comprising perturbation response profiles for one or more diseases and/or therapies, and kits for determining levels of disease states and/or therapeutic effects according to the methods of the invention.
A method of determining a level of one or more disease states in a subject, said method comprising determining, for each of said disease states, an interpolated response profile, from interpolated perturbation response profiles correlated to levels of each of said disease states, for which similarity is greatest between a diagnostic profile and said one or a combination of said determined interpolated response profiles, said diagnostic profile having been obtained by a method comprising measuring a first plurality of cellular constituents in one or more cells of said subject, and wherein said interpolated response profiles are the products of a method comprising
A method of determining a level of effect of one or more therapies upon a subject having a disease state, said method comprising determining, for each of said therapies, an interpolated response profile, from interpolated perturbation response profiles correlated to levels of effect of each of said therapies, for which similarity is greatest between a diagnostic profile and said one or a combination of said determined interpolated response profiles, said diagnostic profile having been obtained by a method comprising measuring a first plurality of cellular constituents in one or more cells of said subject, and wherein said interpolated response profiles are the products of a method comprising:
A computer system for determining a level of one or more disease states in a subject comprising a processor and a memory coupled to said processor, said memory encoding one or more programs, said one or more programs causing said processor to perform a method comprising determining, for each of said disease states, an interpolated response profile, from interpolated perturbation response profiles correlated to levels of each of said disease states, for which similarity is greatest between a diagnostic profile and said one or a combination of said determined interpolated response profiles, said diagnostic profile having been obtained by a method comprising measuring a first plurality of cellular constituents in one or more cells of said subject, and wherein said interpolated response profiles are the products of a method comprising
A computer system for determining a level of effect of one or more therapies upon a subject comprising a processor and a memory coupled to said processor, said memory encoding one or more programs, said one or more programs causing said processor to perform a method comprising determining, for each of said therapies, an interpolated response profile from interpolated perturbation response profiles correlated to levels of effect of each of said therapies, for which similarity is greatest between a diagnostic profile and said one or a combination of said determined interpolated response profiles, said diagnostic profile having been obtained by a method comprising measuring a first plurality of cellular constituents in one or more cells of said subject, and wherein said interpolated response profiles are the products of a method comprising:
A kit for determining a level of one or more disease states in a subject comprising a solid phase containing on its surface a plurality of nucleic acids of known, different sequences, each at a known location on said solid phase, each nucleic acid capable of hybridizing to an RNA species or cDNA species derived therefrom, wherein said RNA species are known to be increased or decreased at different levels of said disease states, said plurality substantially excluding nucleic acids capable of hybridizing to RNA species that are not so increased or decreased.
A kit for determining a level of effect of one or more therapies in a subject comprising a solid phase containing on its surface a plurality of nucleic acids of known, different sequences, each at a known location on said solid phase, each nucleic acid capable of hybridizing to an RNA species or cDNA species derived therefrom, wherein said RNA species are known to be increased or decreased at different levels of said effect of said therapies, said plurality substantially excluding nucleic acids capable of hybridizing to RNA species that are not so increased or decreased.
A kit for determining a level of one or more disease states in a subject comprising:
A kit for determining a level of effect of one or more therapies in a subject comprising:
A database comprising response profiles for one or more disease states or therapies wherein said database is in electronic form, wherein said response profiles are obtained by measuring a plurality of cellular constituents in a cell or cells of one or more analogous subjects at a plurality of levels for each of said disease states or effects of said therapies.
Dependencies are inferred from the claim text.