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US 2001/0012965 A1Published application
In vitro production of transplantable cartilage tissue
AssigneeRush-Presbyterian-St. Luke's Medical Center
InventorsKoichi Masuda, Eugene J-M. A. Thomar, Michael Hejna
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Abstract
The present invention is directed to a transplantable cartilage matrix and a method for its in vitro production.
Claims27
A method for the production of transplantable cartilage matrix, the method comprising:
- isolating chondrogenic cells;
- culturing chondrogenic cells for an amount of time effective for allowing formation of a chondrogenic cell-associated matrix;
- recovering the chondrogenic cells with the cell-associated matrix;
- and
- culturing the chondrogenic cells with the cell-associated matrix on a semipermeable membrane in the presence of growth factor for a time effective for allowing formation of a cartilage matrix.
2 more elements
A cohesive carilage matrix produced by a method comprising:
- isolating chondrogenic cells;
- culturing chondrogenic cells for an amount of time effective for allowing formation of a chondrogenic cell-associated matrix;
- recovering the chondrogenic cells with the cell-associated matrix;
- and
- culturing the chondrogenic cells with the cell-associated matrix on a semipermeable membrane in the presence of growth factor for a time effective for allowing formation of a cohesive cartilage matrix.
2 more elements
A cohesive cartilage matrix comprising:
- at least about 5 mg/cc3 aggrecan;
- collagen types II, IX and XI, and hyaluronan;
- wherein the ratio of aggrecan to hyaluronan is about 10:1 to about 200:1, and the ratio of aggrecan to collagen is about 1:1 to about 10:1.
A method for the surgical repair of cartilage damage comprising:
- producing a transplantable cartilage matrix;
- and
- surgically implanting the cartilage matrix.
Dependencies are inferred from the claim text.