Light microscopy method, device and computer program product

A device comprising:

• a light microscope which is configured to acquire first light microscopic data of a sample in a first acquisition mode and to acquire second light microscopic data of the sample in a second acquisition mode;
• and
• a processor which is configured to recognize an object in the sample from the first light microscopic data using a first artificial intelligence method and to assign the object to an object class,
• wherein the processor is further configured to assign the object to a subcategory of the object class based on the second light microscopic data using the first artificial intelligence method or a second artificial intelligence method,
• wherein the device is configured such that third three-dimensional light microscopic data of the sample are acquired between acquisition of the first light microscopic data and the second light microscopic data, wherein a partial region of the recognized object is selected based on the third light microscopic data, and wherein the second light microscopic data are acquired from the selected partial region of the object.