US 12,768,841 B2 · Compositions and methods for polypeptide analysis

Abstract

Aspects of the application relate to methods and systems for obtaining information regarding multiple amino acids in a polypeptide based on binding interactions between the polypeptide and one or more amino acid recognizers. Kinetic signature information may be obtained from a series of signal pulses indicative of a series of binding events between one or more amino acid recognizers and an amino acid of a polypeptide (e.g., a terminal amino acid, an internal amino acid). The kinetic signature information (e.g., pulse duration, interpulse duration, recognition segment (RS) duration, intersegment duration) may be used to determine one or more chemical characteristics (e.g., identity, modification) of multiple amino acids of the polypeptide.

Claims18

Dependent claims

A method for determining chemical characteristics of a polypeptide, comprising:

contacting a polypeptide with one or more amino acid recognizers, wherein the one or more amino acid recognizers comprise a first set of one or more amino acid recognizers that bind to the polypeptide;
detecting a first series of signal pulses indicative of a first series of binding events between a first type of amino acid recognizer of the first set of one or more amino acid recognizers and the polypeptide, wherein the first series of binding events occur while the same terminal amino acid is present at a terminus of the polypeptide;
and
determining at least one chemical characteristic of a first set of at least two amino acids of the polypeptide based on at least one characteristic of the first series of signal pulses.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

18/145,815Filed 22 Dec 2022 · us provisional application

Priority

22 Dec 2021

Published

US 2023/0213527 A16 Jul 2023

Granted

20 Oct 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 33/6818
General methods of protein analysis not limited to specific proteins or families of proteins: sequencing of polypeptides
G01N 33/6893
Involving proteins, peptides or amino acids: related to diseases not provided for elsewhere
G01N 2440/10
Post-translational modifications [PTMs] in chemical analysis of biological material: acylation, e.g. acetylation, formylation, lipoylation,…
G01N 2440/12
Post-translational modifications [PTMs] in chemical analysis of biological material: alkylation, e.g. methylation, prenylation, farnesylation

Compositions and methods for polypeptide analysis

Life of this patent

Compositions and methods for polypeptide analysis

Life of this patent

Abstract

Claims18