Method for removing protein corona of the surface of nanometer particle

A method for removing protein corona of protein corona modified nanometer particle, the method comprises the following steps:

• subjecting the protein corona modified nanometer particle to ultrasound stimulation to remove the protein corona of the protein corona modified nanometer particle;
• the nanometer particle comprises perfluoropentane;
• the acoustic intensity of the ultrasound stimulation is 2 W/cm2;
• the frequency of the ultrasound stimulation is 3 MHz;
• the duty cycle of the ultrasound stimulation is 50%;
• the duration time of the ultrasound stimulation is 5 min;
• the nanometer particle is ligand-modified liposome, the ligand-modified liposome is prepared by the following method, which comprises:
• (i) dissolving 2.8-3.2 mg of DPPC, 2.8-3.2 mg of DSPE-PEG-ligand, 1.8-2.2 mg of DSPE-PEG and 1.8-2.2 mg of drug in an organic solvent in round bottom flask, and removing the organic solvent by rotary evaporation under reduced pressure to obtain a lipid film in round bottom flask, wherein the ligand is RGD polypeptide, the amino acid sequence of the RGD polypeptide is CRGDKGPDC, the DPPC refers to 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DSPE-PEG refers to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)], the drug is gemcitabine elaidate having the following structure
• CP4126
• (ii) cooling the lipid film to 2-6° C. and adding 90-110 μL of perfluoropentane to immerse the lipid film, then adding 4.5-5.5 ml of buffer solution to hydrate, stirring at 2-6° C., and then stirring at 28-32° C. in the open round bottom flask to obtain the ligand-modified liposome.