Genetically engineered peptide superstructure and microbe for production of isoprene and method of production thereof

A genetically engineered microbe capable of producing isoprene, the genetically engineered microbe comprises:

• a heterologous nucleic acid construct encoding at least a portion of a peptide superstructure, wherein the superstructure comprises:
• a first portion comprising a first binding site configured to catalyze a first chemical reaction, wherein the first reaction converts a substrate to an intermediate;
• and
• a second portion comprising a second binding site configured to catalyze a second chemical reaction, wherein:
• the second portion is covalently linked to the first portion through a linker;
• and
• the second chemical reaction converts the intermediate to isoprene;
• and wherein:
• the first portion comprises isopentenyl diphosphate (IPP) isomerase comprising a sequence of SEQ ID NO:
• 30 or SEQ ID NO:
• 31 or enzymatically active fragment thereof;
• and
• the second portion comprises isoprene synthase (IspS) comprising a sequence of SEQ ID NO:
• 25 or SEQ ID NO:
• 26, or enzymatically active fragment thereof.