Compositions and methods for altering stem length in solanaceae
AssigneeUniversity of Florida Research Foundation, Inc.
InventorTong Geon Lee
Life of this patent
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Abstract
Described are CRISPR constructs and systems that can be used to generate brachytic Solanaceae plants rapidly and efficiently. Also described are methods of introducing a brachytic phenotype into a Solanaceae plant having one or more other desired traits using the described CRISPR constructs and systems to generate loss of function mutations in one or more brachytic loci in the plant.
Claims18
A genetically modified Solanum lycopersicum plant wherein Solyc01g066950 locus and a Solyc01g066970 locus have been genetically modified through the use of a CRISPR/Cas system,
- wherein the Solyc012g066950 locus consists of SEQ ID NO:1 and the Solyc01g066970 locus consists of SEQ ID NO:6;
- and
- wherein the genetic modification results in loss of function or decreased expression of coding sequences comprising SEQ ID NO:2 and SEQ ID NO:7.
A method of genetically modifying a Solanum lycopersicum plant to produce a plant having a brachytic phenotype, the method comprising:
- (a) introducing a CRISPR system into a Solanum lycopersicum plant cell, wherein the CRISPR system comprises:
- (i) one or more RNA-guided DNA endonucleases or one or more nucleic acids encoding the one or more RNA-guided DNA endonucleases;
- and
- (ii) one or more guide RN As or one or more nucleic acids encoding the one or more guide RNAs into a plant cell;
- wherein the one or more RNA-guided DNA endonucleases and the one or more guide RNAs form one or more complexes that target a Solyc01g066950 locus and a Solyc01g066970 locus, and
- wherein the Solyc01g066950 locus consists of SEQ ID NO:
- 1 and the Solyc01g066970 locus consists of SEQ ID NO:6, and
- thereby decreasing expression of or generating loss of function mutations in coding sequences comprising SEQ ID NO:2 and SEQ ID NO:7;
- and
- (b) generating one or more regenerant plants following introducing the CRISPR system into the Solanum lycopersicum plant cell, and
- wherein the plants and/or progeny of the plants comprise the genetic modification resulting in loss of function or decreased expression of coding sequences comprising SEQ ID NO:2 and SEQ ID NO:7.
Dependencies are inferred from the claim text.