Back to results
US 12,767,953 B2Granted
Mogroside compositions and methods of producing same
AssigneeElo Life Systems
InventorsTengfang Huang, Jack Q. Wilkinson, Anna Matthiadis, Devarshi Selote, Matthew Kilgore, Matthew DiLeo, Fayaz Khazi, Kevin Potter, Daniel Stauffer, Todd Rands
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods for producing transgenic plants and other organisms that exhibit increased production of mogroside compounds, in particular mogroside V, and the mogroside compounds, plants and plant parts so produced.
Claims15
A recombinant DNA molecule comprising:
- a) a first polynucleotide sequence that encodes a cytochrome P450 polypeptide having at least 91% sequence identity to SEQ ID NO:2 or at least 95% sequence identity to SEQ ID NO:
- 86;
- b) a second polynucleotide sequence that encodes a cucurbitadienol synthase polypeptide having at least 90% sequence identity to SEQ ID NO:5;
- c) a third polynucleotide sequence that encodes a cytochrome P450 polypeptide having at least 90% sequence identity to SEQ ID NO:7, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31 or SEQ ID NO:33;
- d) a fourth polynucleotide sequence that encodes a uridine phosphorylase-dependent glycosyltransferase polypeptide having at least 90% sequence identity to SEQ ID NO:9;
- e) a fifth polynucleotide sequence that encodes a uridine phosphorylase-dependent glycosyltransferase polypeptide having at least 90% sequence identity to SEQ ID NO:11;
- f) a sixth polynucleotide sequence that encodes a squalene epoxidase polypeptide having at least 90% sequence identity to SEQ ID NO:13;
- g) a seventh polynucleotide sequence that encodes an epoxy hydrolase polypeptide having at least 90% sequence identity to SEQ ID NO:15;
- and
- h) an eighth polynucleotide sequence that encodes a truncated 3-hydroxy-3-methylglutaryl-CoA reductase polypeptide having at least 95% sequence identity to SEQ ID NO:
- 273;
- wherein the first, second, third, fourth, fifth, sixth, seventh and/or eighth polynucleotide sequence is operably linked to a heterologous promoter.
9 more elements
Dependencies are inferred from the claim text.