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US 12,767,942 B2Granted
Method for purification of botulinum toxin complex with improved purification yield
AssigneeInibio Co., Ltd.
InventorsChung Sei Kim, Young Jun Song
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Abstract
The present invention relates to a method for purification of botulinum toxin with improved purification purity and yield. When the method for purification of botulinum toxin of the present invention is used, botulinum toxin can be purified with a very high yield.
Claims4
A method for purification of botulinum toxin complex comprising the following steps:
- (a) a step of preparing a strain culture solution producing botulinum toxin;
- (b) a first purification step of purifying the prepared culture solution using a mixed-mode anion exchange resin;
- and
- (c) a second purification step of purifying the culture solution that has passed the first purification step using a mixed-mode cation exchange resin,
- wherein the mixed-mode anion exchange resin and the mixed-mode cation exchange resin have mixed-mode selectivity in which hydrophobic interaction and ionic interaction are mixed.
2 more elements
Dependencies are inferred from the claim text.