Method for preparing animal tissue-derived biomaterial, animal tissue-derived biomaterial prepared thereby, and 3D printing method using same

A method for preparing an animal tissue-derived biomaterial, the method comprising:

• decellularizing a tissue to remove cells from the tissue;
• liquefying an extracellular matrix of the decellularized tissue using an acidic protease;
• filtering the decellularized extracellular matrix solution obtained through the liquefying;
• and
• sterilizing the resulting mixture obtained through the filtering, wherein the filtering comprises:
• filtering the decellularized extracellular matrix solution through a 70 to 100 kDa ultra-filtration membrane to obtain a concentrated solution containing collagen, elastin and glycosaminoglycan, and a filtrate containing growth factors, cytokines and the acidic protease;
• filtering the concentrated solution through a 0.1 to 5 kDa ultra-filtration membrane to obtain a primary solution;
• filtering the filtrate to remove the acidic protease contained in the filtrate to obtain a secondary solution;
• and
• mixing the primary solution and the secondary solution.