Methods and compositions for T-cell coculture potency assays and use with cell therapy products

A method of determining the potency and/or functionality of a tumor infiltrating lymphocyte (TIL) product for treatment of a subject, the method consisting of assessing the TIL product using a potency and identity assay matrix;

• wherein the potency and identity assay matrix consists of a co-culture assay with U937 target cells wherein IFN-γ release by TIL cells from the TIL product in response to T cell receptor (TCR)-Human Leukocyte Antigen (HLA) engagement is measured, a bead-based assay wherein IFN-γ release by TIL cells from the TIL product is measured, a flow cytometry assay wherein LAG3+ cell content is measured, a flow cytometry assay wherein KLRG1+ cell content is measured, a flow cytometry and cell counter assay wherein the sum of CD4+ cell content and CD8+ cell content is measured, a flow cytometry and cell counter assay wherein total viable TCM and TEM cell population is measured, and a flow cytometry assay wherein CD45+/CD3+ cell content is measured,
• wherein the TIL product comprises CD8+ TILs and CD4+ TILs.