Back to results
US 12,767,695 B2Granted
Method for producing human professional antigen-presenting cells
AssigneesAGC Inc., National Cancer Center Japan
InventorsYuichiro Hagiya, Yasushi Uemura, Kyoko Fukuda, Tatsuaki Iwama
Life of this patent
Estimating the term…
Abstract
A method for producing a professional antigen-presenting cell, including inducing expression of c-MYC, BMI1, and MDM2 in a myeloid cell (MC) to obtain a proliferative myeloid cell (pMC), and inducing expression of GM-CSF and/or M-CSF in the pMC to obtain a professional antigen-presenting cell (pAPC). The myeloid cell is a myeloid cell differentiated from a pluripotent stem cell.
Claims5
A method for producing a professional antigen-presenting cell (pMC), comprising
- differentiating myeloid cells (MC) from a pluripotent stem cell by the following method (i) or (ii):
- (i) performing a double-laver culture of embryoid body (EB) induced from the pluripotent stem cells on feeder cells in a medium containing vascular endothelial growth factor (VEGF), and further culturing same in a medium containing VEGF, stem cell factor (SCF), and thrombopoietin (TPO), or
- (ii) performing a monolayer culture of an embryoid body (EB) induced from the pluripotent stem cells in a medium containing bone morphogenetic protein 4 (BMP-4), VEGF, and SCF without feeder cells, and further culturing same in a medium containing VEGF, TPO, and granulocyte-macrophage colony-stimulating factor (GM-CSF),
- inducing expression of Myc proto-oncogene protein (c-MYC), polycomb complex protein BMI-1 (BMI1), and E3 ubiquitin-protein ligase Mdm2 (MDM2) in a myeloid cell (MC) to obtain a proliferative myeloid cell (pMC), and
- inducing expression of GM-CSF and/or Macrophage colony-stimulating factor (M-CSF) in the pMC to obtain a professional antigen-presenting cell (pAPC).
2 more elements
A method for producing a professional antigen-presenting cell, comprising
- (i) seeding pluripotent stem cells in a container for cell mass-forming culture that has fine spheroid wells at a bottom, and does not have a flat surface between adjacent spheroid wells to form an embryoid body (EB),
- (ii) obtaining myeloid cells (MC) from the embryoid body (EB) by the method of the following (ii)-1 or (ii)-2:
- (ii)-1 performing a double-layer culture of the embryoid body on feeder cells in a medium containing VEGF, and further culturing same in a medium containing VEGF, SCF, and TPO, or
- (ii)-2 performing a monolayer culture of the embryoid body in a medium containing BMP-4, VEGF, and SCF without feeder cells, and further culturing same in a medium containing VEGF, TPO, and GM-CSF,
- (iii) inducing expression of c-MYC, BMI1, and MDM2 in the myeloid cells (MC) obtained in (ii) to obtain proliferative myeloid cells (pMC), and
- (iv) inducing expression of GM-CSF and/or M-CSF in the proliferative myeloid cells (pMC) obtained in (iii) to obtain professional antigen-presenting cells (pAPC).
3 more elements
Dependencies are inferred from the claim text.