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US 12,767,690 B2Granted
Nucleic acid-binding protein
AssigneeJapan Science and Technology Agency
InventorsYasushi Okada, Daisuke Ino, Kazuho Ikeda
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AssigneeJapan Science and Technology Agency
InventorsYasushi Okada, Daisuke Ino, Kazuho Ikeda
Estimating the term…
The present invention provides a novel modified protein for detecting a chromatin open structure more sensitively and easily than the prior art as a new detection tool related to gene expression. The present invention relates to a nucleic acid-binding protein comprising a DNA-binding domain in which 3 or more TAL-repeats are repeatedly linked, wherein the nucleic acid-binding protein binds in a base sequence-independent manner, and a method for fluorescently labeling open chromatin in living cells, comprising a step of introducing a gene encoding a nucleic acid-binding protein into a living cell, wherein the nucleic acid-binding protein is a protein in which a DNA-binding domain in which 3 or more TAL-repeats are repeatedly linked and a fluorescent protein are directly or indirectly bound, and the DNA-binding domain binds to a nucleic acid in a base sequence-independent manner.
A nucleic acid-binding protein comprising a DNA-binding domain, wherein the DNA-binding domain consists of 3 or more and less than 35 Transcription Activator-Like-repeats (TAL-repeats) that are repeatedly linked, and wherein the Repeat Variable Di-residue (RVD) sequences of the TAL-repeats in the DNA-binding domain consist of a sequence pair selected from the following groups:
Dependencies are inferred from the claim text.