Magnetic sorting microfluidic chip and manufacturing method therefor

A magnetic sorting microfluidic chip, comprising:

• a substrate,
• a chip model material layer,
• a micro-channel unit and
• a magnetic sorting unit, wherein
• the chip model material layer is disposed on the substrate, and the micro-channel unit and the magnetic sorting unit are both disposed in the chip model material layer;
• the micro-channel unit comprises a sorting channel, and a first magnetic pole channel and a second magnetic pole channel which are symmetrically arranged on a left side and a right side;
• the first magnetic pole channel includes a first magnetic pole channel inlet and a first micro-channel filtration column;
• the second magnetic pole channel includes a second magnetic pole channel inlet and a second micro-channel filtration column;
• the first and second micro-channel filtration columns are configured to block the ferromagnetic powder;
• the first magnetic pole channel and the second magnetic pole channel are provided and shared with a common magnetic pole channel outlet;
• the first and second magnetic pole channels are used for forming two magnetic pole arrays having opposite magnetic polarities;
• and the magnetic polarities of the magnetic pole arrays inside the first magnetic pole channel and the second magnetic pole channel are opposite;
• the sorting channel is provided with a plurality of sorting channel inlets and a plurality of sorting channel outlets and is separated from the first magnetic pole channel and the second magnetic pole channel;
• the sorting channel inlets comprise a particle inlet and a sheath flow inlet;
• particles or cells of no less than two different sizes are introduced from the particle inlet, and the particles or cells are focused by a sheath flow on a side close to the first and second magnetic pole channels;
• liquid carriers introduced from the particle inlet and the sheath flow inlet are both magnetic solutions;
• and are used for sorting non-magnetic particles or cells;
• the magnetic sorting unit comprises a first and a second permanent magnets made of material having remanence greater than or equal to 0.5 Tesla, a first and a second high-permeability alloys, and a first and a second magnetic pole arrays disposed in the first and second magnetic pole channels, wherein the first and second high-permeability alloys are configured to conduct magnetic fields of the first and second permanent magnets to the first and second magnetic pole arrays, so that the magnetic pole arrays generate two magnetic fields having high intensity, high gradient, and opposite polarities on left and right positions of a same side of the sorting channel, and thus the sorting channel sorts, according to sizes, particles to be processed into different sorting channel outlets;
• and
• wherein the common magnetic pole channel outlet is disposed in a middle of the first and the second permanent magnets, the first and second magnetic pole channel on the left and right sides.