US 12,766,510 B2 · Microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber

Abstract

A microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber is disclosed. For example, the microfluidics device includes a pair of substrates separated by a gap and thereby forming a reaction (or assay) chamber therebetween. A field of actuatable surface-attached posts (e.g., magnetically responsive microposts) is provided on one or both of the substrates. The surface-attached posts are functionalized with capture beads. Additionally, methods are provided of functionalizing the surface-attached posts with the capture beads. Additionally, methods are provided of using the surface-attached posts that are functionalized with capture beads in a microfluidics device for binding a target of interest. Further, a bead spraying system and method is provided for spraying magnetically responsive and/or non-magnetically responsive beads atop and/or among a field of surface-attached microposts for use in a microfluidic device.

Claims17

Dependent claims

A microfluidic cartridge comprising:

a. a housing forming a reaction chamber;
b. a field of actuatable surface-attached microposts provided on an interior surface of the housing and extending into the reaction chamber, wherein the actuatable surface-attached microposts are configured to move when actuated;
and
c. beads attached to the microposts;
and
wherein the beads comprise a core that is covered by a polymer shell and the polymer shell provides a surface for a subsequent functionalization reaction or reactions, and the core further comprises a magnetically-responsive material.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/776,797Filed 16 Nov 2020 · us provisional application

Priority

15 Nov 2019

Published

US 2022/0395834 A115 Dec 2022

Granted

20 Oct 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 2200/0668
Handling flowable solids, e.g. microscopic beads, cells, particles: trapping microscopic beads
B01L 2300/0877
Configuration of multiple channels and/or chambers in a single devices: flow chambers
B01L 2300/123
Specific details about materials: flexible; Elastomeric

Microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber

Life of this patent

Microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber

Life of this patent

Abstract

Claims17