Methods, apparatuses, and systems for the treatment of disease states and disorders

A method of treating cancer of a subject in need thereof, the method comprising:

• advancing a device comprising an elongate shaft and an electrode into the subject having the cancer, the electrode electrically coupled to a generator programmed with an energy delivery algorithm configured to generate an electric signal having a waveform comprising a plurality of energy packets with a rest period between each energy packet, wherein each energy packet comprises a plurality of biphasic pulses;
• penetrating tissue with the device to enter an embedded targeted region in the subject;
• and
• applying pulsed electric field energy via the electrode to the embedded targeted region based on the electric signal generated by the generator to target cancer cells to induce cell death of the cancer cells in a non-thermal manner while maintaining an extracellular matrix in the embedded target region, thereby treating the cancer of the subject,
• wherein the electric signal comprises a frequency and a voltage, and wherein an effect of the frequency inversely balances an effect of the voltage so as to target the cancer cells.