Gene leading to ToBRFV resistance in S. lycopersicum
AssigneeRijk Zwaan Zaadteelt En Zaadhandel B.V.
InventorsJonathan Kalisvaart, Daniël Johannes Wilhelmus Ludeking, Alwin Johannes Marinus Roovers
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Abstract
The invention relates to a Solanum lycopersicum plant that is resistant to ToBRFV, which plant comprises a QTL on chromosome 8 between SEQ ID NO: 1 and 4. The presence of the QTL on chromosome 8 is identified by use of at least one of the markers selected from the group consisting of the SNP presented in SEQ ID NOS: 1 to 42. The QTL is present in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 43637.
Claims5
A method for identifying a QTL on chromosome 8 that leads to Tomato brown rugose fruit virus (ToBRFV) resistance in a plant, comprising identifying a marker in the plant, wherein:
- the plant is a Solanum lycopersicum (S. lycopersicum) plant, or a Solanum pimpinellifolium plant, or a plant of another Solanum species that is sexually compatible with S. lycopersicum;
- and
- the marker is located between SEQ ID NO:
- 1 and SEQ ID NO:
- 42, and comprises SEQ ID NO:
- 28 and/or a GGC deletion at the position corresponding to positions 312-314 of SEQ ID NO:
- 47.
A method for selecting a Tomato brown rugose fruit virus (ToBRFV) resistant plant, comprising identifying the homozygous presence of a QTL on chromosome 8 of a plant, and selecting the plant that homozygously comprises said QTL as a ToBRFV resistant plant, wherein:
- the plant is a Solanum lycopersicum (S. lycopersicum) plant, or a Solanum pimpinellifolium plant, or a plant of another Solanum species that is sexually compatible with S. lycopersicum;
- and
- identifying comprises identification within the QTL of the presence of SEQ ID NO:
- 28 and/or identification within the QTL of a GGC deletion at the position corresponding to positions 312-314 of SEQ ID NO:
- 47.
A method of selecting a Solanum lycopersicum plant or plant part that exhibits increased resistance to Tomato brown rugose fruit virus (ToBRFV), comprising:
- a) identifying in a Solanum lycopersicum plant or plant part by genetic analysis the homozygous presence of a QTL on chromosome 8 located between SEQ ID NO:
- 1 and SEQ ID NO:
- 42, wherein identifying comprises identification within the QTL of the presence of SEQ ID NO:
- 28, and/or identification within the QTL of a GGC deletion at the position corresponding to positions 312-314 of SEQ ID NO:
- 47;
- and
- b) selecting a plant or plant part from a) that comprises said QTL homozygously, optionally testing the selected plant or plant part for increased resistance to ToBRFV as compared to a Solanum lycopersicum plant or plant part that does not comprise said QTL homozygously, wherein the selected plant or plant part does exhibit increased resistance to ToBRFV as compared to a Solanum lycopersicum plant or plant part that does not comprise said QTL homozygously.
Dependencies are inferred from the claim text.