Microfluidic immunoassay chip and microfluidic line immunoassay method
AssigneeShenzhen Yhlo Biotech Co., Ltd.
InventorsYanping Zheng, Xiaoyu Cheng, Daoshun Wei, Chungen Qian, Kunhui Hu
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Abstract
The present disclosure relates to a microfluidic immunoassay chip and a microfluidic line immunoassay method. The microfluidic immunoassay chip includes a loading cell, a reaction and detection cell, a washing cell, an enzyme storing cell, a substrate cell, and a termination cell, and a waste liquid cell. A detection membrane strip is disposed in the reaction and detection cell and coated with a capture antigen or a capture antibody.
Claims20
A microfluidic immunoassay chip, comprising at least one detection unit and having a rotation center, wherein the detection unit comprises:
- a loading cell provided with a loading hole;
- a reaction and detection cell being in communication with the loading cell, the reaction and detection cell being provided with a detection membrane strip coated with a capture antigen or a capture antibody;
- a washing cell configured to be pre-filled with a buffer solution, the washing cell being in communication with the reaction and detection cell;
- an enzyme storing cell configured to be pre-filled with an enzyme labelled antibody, the enzyme storing cell being in communication with the reaction and detection cell;
- a substrate cell configured to be pre-filled with a reaction substrate for the enzyme labelled antibody, the substrate cell being in communication with the reaction and detection cell;
- a termination cell configured to be pre-filled with a reaction terminating liquid, the termination cell being in communication with the reaction and detection cell, the reaction and detection cell being configured to receive the reaction terminating liquid released from the termination cell;
- and
- a waste liquid cell configured to receive a waste liquid discharged from the reaction and detection cell, the waste liquid cell being in communication with the reaction and detection cell;
- wherein the reaction and detection cell is further away from the rotation center than the loading cell, the washing cell, the enzyme storing cell, the substrate cell, and the termination cell, and is closer to the rotation center than the waste liquid cell.
A microfluidic immunoassay chip, comprising at least one detection unit and having a rotation center, wherein the detection unit comprises:
- a loading cell provided with a loading hole;
- a reaction and detection cell being in communication with the loading cell, the reaction and detection cell being provided with a detection membrane strip coated with a capture antigen or a capture antibody;
- a washing cell configured to be pre-filled with a buffer solution, the washing cell being in communication with the reaction and detection cell, the reaction and detection cell being configured to receive the buffer solution released from the washing cell;
- an enzyme storing cell configured to be pre-filled with an enzyme labelled antibody, the enzyme storing cell being in communication with the reaction and detection cell;
- a substrate cell configured to be pre-filled with a reaction substrate for the enzyme labelled antibody, the substrate cell being in communication with the reaction and detection cell;
- a termination cell configured to be pre-filled with a reaction terminating liquid, the termination cell being in communication with the reaction and detection cell, the reaction and detection cell being configured to receive the reaction terminating liquid released from the termination cell;
- and
- a waste liquid cell configured to receive a waste liquid discharged from the reaction and detection cell, the waste liquid cell being in communication with the reaction and detection cell;
- wherein a side of the reaction and detection cell adjacent to the rotation center is further away from the rotation center than the loading cell, the washing cell, the enzyme storing cell, the substrate cell, and the termination cell, and a side of the reaction and detection cell away from the rotation center is closer to the rotation center than the waste liquid cell.
Dependencies are inferred from the claim text.